* Note:

Criteria for mitotic figure: 1) absence of nuclear membrane, 2) chromosomal (radiating) alignment, and 3) intracytoplasmic.

The mitotic rate is based on  per 5 mm2 on the histologic tissue section on glass slide. In most new microscopes, this

approximately is 21 hpf . Below is the calculation.

       Diameter of view field (mm) = 22 / 40 = 0.55, where 22 is the field number of eyepiece, and 40 = objective mag.

       Area of view at 40x (mm2) = Pi x R2 = 3.1416 x (0.55/2)2 = 0.23758294793 mm

       Number of fields to be 5 mm2 =  5 / 0.23758294793 = 21 fields (at 40 X)