Immunofluorescence (IF) studies are part of the work-up for vesiculobullous (blistering) disorders and a few other conditions. They require special processing.
If a physician requests IF studies, the biopsy must arrive in transport media (usually called Michel’s media or Zeus’s media) or without fixative. IF studies cannot be performed on formalin-fixed tissue. If the biopsy arrives in saline or wrapped in gauze, place it in Michel’s media (kept in the refrigerator).
The specimen will usually be a punch biopsy.
If IF is being requested in the evaluation of a non-blistering condition (e.g. vasculitis), you may be asked to bisect the specimen, reserving half for routine processing and half for IF. Do not bisect a biopsy from a patient with a vesiculobullous disorder!
Received in Michel’s media, labeled _____ and “_____”, is a __ x __ cm punch biopsy of (color) skin. There is a __ cm _____ (location, color, border) _____ (lesion-- papule, macule, ulcer) on the epidermal surface. Entirely submitted for immunofluorescence studies in cassette #__.